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Acute toxicity curve for AgNP nano ink in 11 exposure vessels. EC 50 values are in μL/L. Fifteen 4−day−old daphnids per replicate of each concentration tested were exposed to AgNPs nano ink for 24 h. Data represent average ± SD (N = 5). Using the four-parameter logistic (4PL) model, the EC 50 values were calculated.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Miniaturizing Nanotoxicity Assays in Daphnids

doi: 10.3390/ani14142046

Figure Lengend Snippet: Acute toxicity curve for AgNP nano ink in 11 exposure vessels. EC 50 values are in μL/L. Fifteen 4−day−old daphnids per replicate of each concentration tested were exposed to AgNPs nano ink for 24 h. Data represent average ± SD (N = 5). Using the four-parameter logistic (4PL) model, the EC 50 values were calculated.

Article Snippet: The EC values were calculated and a concentration of 0.5 μL nano ink/L (derived from the data of the toxicity curves) was used as sub-lethal exposure concentration for the enzyme activity and feeding rate exposures of 24 h. Silver nanoparticle ink was purchased from Sigma-Aldrich to conduct the exposure experiments (Product No. 719048).

Techniques: Concentration Assay

The impact of vessel on mortality. Points in the figure correlate to each exposure vessel with axes used to plot the characteristics of the vessel. Colour intensity represents the amount of AgNP nano ink (mg/daphnid in the exposure vessel) for the EC 50 . A supplementary version of this image with free rotation is provided.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Miniaturizing Nanotoxicity Assays in Daphnids

doi: 10.3390/ani14142046

Figure Lengend Snippet: The impact of vessel on mortality. Points in the figure correlate to each exposure vessel with axes used to plot the characteristics of the vessel. Colour intensity represents the amount of AgNP nano ink (mg/daphnid in the exposure vessel) for the EC 50 . A supplementary version of this image with free rotation is provided.

Article Snippet: The EC values were calculated and a concentration of 0.5 μL nano ink/L (derived from the data of the toxicity curves) was used as sub-lethal exposure concentration for the enzyme activity and feeding rate exposures of 24 h. Silver nanoparticle ink was purchased from Sigma-Aldrich to conduct the exposure experiments (Product No. 719048).

Techniques: